A Comparison of TatC subunit in gram-positive and gram-negative bacteria

Order Description

The subject topic is bioinformatics. I need to compare TatC subunit from gram-positive and gram-

negative bacteria in order to investigate differences in amino acid sequences and structure.

The gram positive bacteria that need to be used are:

Streptomyces scabies WP_059082011.1, Corynebacterium uterequi AKK11079.1, Corynebacterium

glutamicum AMA00156.1, Staphylococcus aureus KFA45045.1, Bacillus amyloliquefaciens KDN92708.1 and

Bacillus subtilis KFK77267.1.

The Gram-negative bacteria are; Aquifex aerolicus WP_010880807.1, Stenotrophomonas maltophilia

AIL06264.1, Escherichia coli CCJ46466.1, Rickettsia prowazekii AMS12659.1, Rhizobium leguminosarum

KZA97414.1 and Shewanella oneidensis WP_011073887.1

The databases from which i need the information from are:

Clustal Omega,
CLustal W2,
Phylogenetic tree,
Swiss model,
protter 1.0

My hypothesis is:

Gram-positive and Gram-negative bacteria have substantial structural and regional differentiation

in TatC subunits and both of them contain different properties.

Aim is:

Whether this study was able to distinguish both the TatC regions and TatC subjects from Gram

negative and Gram positive TatC bacteria.
Objectives include:

To choose up to six Gram negative TatC bacteria and six Gram positive TatC bacteria in order to

compare and identify the amino acid sequences regions. (listed above).

? By means of several bioinformatics software tools such as BLAST to determine if the sequences

were conservative or non-conservative.

? Whether the residues displayed polarity or non-polarity, also if they were positively charged or

negatively charged.

? Finally, compare the structure, characteristics and properties of both Gram positive and Gram

negative bacteria as well as in what way these exist different and how they are similar to one