Broad overview of D. melanogaster as a model organism.
Discuss the particular biological processes/function associated with the wildtype gene.
Explain how a mutation in your gene would affect its function
Find a human ortholog of your gene and do briefly explain if it's associated with any diseases or disorders. If you can't find an ortholog in humans, find any other species.
Excplicitly state the objectives of the project and briefly mention the broad steps you've done (save the details for the methods section)
Gives a 3-4 sentence summary of the methods, so the reader knows what to expect
Describes how flies were cultured and manipulated. Only include enough information for reproducibility (media, incubation temp, etc)
Explains how each cross was conducted along with the purose for each cross. Everytime you present a technique or procedure, briefly mention the purpose of that technique. For exapmple, you may say "in order to determine the chromosomal location of ebony gene, a series of mapping crosses were done where we crossed a by b and c by d.