Diagnosis of Mycobacterium tuberculosis

Diagnosis of Mycobacterium tuberculosis

Order Description

1- How to detect Mycobacterium tuberculosis
2-Include the use of Xpert MTB/RIF
3- Talk about Multidrug resistant tuberculosis and extensively drug resistant tuberculosis
4-talk about Hain assay
5- whole genome sequencing
6-is the treatment going to be successful? include personalized medicine and early evaluation of treatment (I will upload some references for that)
7-Drug action and bacterial population use Mitchison 1979 paper for that and also include these two paper: profiling persistent tubercle bacilli from patient sputa
during therapy predicts early drug efficacy and cytological and transcript analyses reveal fat and lazy persister like bacilli also talk about the indifferent between
day 14 and prechemotherapy using honeborne paper. And the implications in vivo using SH Gillespie paper
7-chnges in the gene expression: a measure of treatment efficacy
8- predicting outcome? host and bacterial biomarkers
Host biomarkers include clinical-Radiological and immunological
Mycobacterial biomarkers include sputum smear, EBA, sputum culture conversion and speculative markers serial sputum colony counts, time to culture conversion, whole
blood bacterial assay and molecular markers
9- Bacterial load include
cavitation is associated with increased bacterial load (perin paper)
measuring bacterial load by culture
decline of bacilli following treatment
smear is a poor predictor of culture
10- can we use PCR to quantify using Robert blakmore paper
11- the use of digital PCR to improve the application of quantitative molecular diagnostic method
12- molecular bacterial load assay