Discussion two different cases
to different cases each of the maximum 500 for discussion part
CASE DETAILS: (2.5 marks)
Microbiologist-in-charge: your name (provide partner’s name also)
Patient Name: the patient’s name
Type of specimen: e.g. Urine
Microscopy: This is of original specimen; please copy as written in manual, don’t include the
Write “N/A” if none performed/supplied.
LAB RESULTS: (10 marks)
(i) Here, list the organism/s isolated and how much of each organism was grown: (e.g. Heavy
growth of E. coli).
? EXCEPT FOR URINE, where you have to say the number per ml (e.g. 7.2 X 104 E.
coli/ml but if more than 105, then e.g. >105 E. coli/ml).
(ii) If antimicrobial susceptibilities performed they are reported here, in a table format.
These allow the doctor to decide on the appropriate treatment.
(iii) Place all your results here: in a table format, set out in sequence as they were done.
? colony morphology
? growth conditions
? Gram stain
? all ID tests (list controls used)
? include zone diameters for antimicrobial susceptibility tests for isolates and controls
DISCUSSION/REPORT: (No more than 500 words)
(i) Discuss your results and include the following in your discussion: (25 marks)
? How did you arrive at your identification?
? Importance of pure cultures, any problems you experienced.
? Suggestions for improving the procedure you used.
? Suggestions for any other media that could be used or tests that could be
? Were any problems experienced with susceptibility tests?
? Did looking at one week old, refrigerated, cultures affect the results?
? Any other thoughts?
? Does the culture result correlate with the specimen’s original microscopy?
? Is/are the isolated organism/s significant? i.e. suggestive of infection or is it
normal flora, skin contaminant, environmental contaminant, etc., give a
? Is/are the organism/s normally a pathogen, an opportunistic pathogen, part of
the normal flora?
? What infections/diseases can it cause? List only a couple.
? If it was to be to be treated, list one antimicrobial that could be used.
(ii) Compare your report with the corresponding real-world report provided and: (10marks)
? identify at least two differences (in tests/techniques used; results obtained);
? discuss the differences, explaining possible reasons for them.
REFERENCES: (2.5 marks)
At least five references in addition to the lab manual (authorship and publication
details on the inside cover).