Microbiology Lab

View the following videos: Enzyme immunoassay (EIA) to detect antigens - Multi-Lingual Captions: https://www.youtube.com/watch?v=70TPrfL_8-M Enzyme-Linked Immunosorbent Assay (ELISA) to Measure Specific Serum Antibodies: https://www.youtube.com/watch?v=RRbuz3VQ100 Describe the steps involved in performing each of the following tests (see questions). 1. Test cellular material collected from the lesions of a patient for the presence of varicella zoster virus (VZV) antigens by direct immunofluorescence. 2. Test cellular material collected from the lesions of a patient for the presence of varicella zoster virus (VZV) antigens by indirect immunofluorescence. 3. Test a patient’s serum for the presence of antibodies against Treponema pallidum (the bacterium that causes syphilis) by immunofluorescence. 4. Use an ELISA (enzyme-linked immunosorbent assay) [or EIA (enzyme immunoassay)] to test a patient’s serum for the presence of antibodies againstmeasles virus.. 5. Use an ELISA (enzyme-linked immunosorbent assay) [or EIA (enayme immunoassay)] to test a sick animal’s serum for the presence of measles virus antigens.