Molecular Spectroscopy

    Fluorescence Questions Molecular Spectroscopy - Week 2 1. Discuss how absorbance spectra are related to fluorescence spectra (emission and excitation). a. How did we use the absorbance spectra in relationship to the fluorescence experiments? i. Remember to include how we utilized the 7 major absorbance peaks in the fluorescence portion of the experiment (Below are absorbance, excitation and emission spectra of pyrene for reference purposes)         2. Describe how you would go about trying to determine which wavelength would yield the greatest fluorescence. Discuss this in terms of both absorbance and fluorescence related experiments. 3. Describe how to conduct an excitation scan. 4. Describe how to conduct an emission scan. 5. What effect does increasing the excitation slit width have on emission spectra and why? 6. What effect does increasing the data collection increments have on the collection of emission spectra and why?