Description:
a lab report about the lab we did 1/24/17. Introduction,purpose in the introduction. Materials and methods, Results and Table 1,2, and graph. So we did air experiment for 1min,2mins,5mins,and 10mins, then we did hypotheses experiment. Q 1; Are there microbes in the air? Q 2; Are there microbes on every surface? Q3; Is the “5 seconds rule ” legitimate? Q4; Can microbes be found o and in human?
Thank you for your help. Materials are 1 Wax pencils, 2 test tube racks, 6 metal inoculation loops, 6 metal inoculating, 2 Bunsen burners and hoses, 10 sterile Cotton swabs( 10 per table), 10 sterile tryptone broth test tubes, 10 sterile agar plates, 3 sterile blood agar plates, 1 waste 500ml Beakers. Experiment 1 are there microbes in the air and if so, which once? So, 1 plate should be labeled 1 minute, another plate 2 minutes, 5 minutes, 10 minutes, 15 minutes. Take the temperature of your incubator and record it in your notebook. 35 degree. Remove your plates from the incubator. Use the colony counter to count how many CFUs of bacteria, yeast, and mold are present and record your data. I just sent you pictures of the instructions of the experiments. The results for the air experiment was 1 minute (Bacteria) 0 ( yeast) 3 (mold) 0. 2 minutes,(bacteria)0 (yeast) 0 (mold) 0. 5 minutes ( bacteria) 0 (yeast)3 (mold)0. 10 minutes, (bacteria) 6 (yeast) 4 (mols) 0. 15 minutes (bacteria) 0 (yeast) 7 (mold) 0. The next experiment was surface. The result was ( phone)= 2 bacteria, 0 yeast, 0 mold. ( Chair)= 7 bacteria, 18 yeast, 1 mold. ( door knob)= 0 bacteria, 0 yeast, 0 mold. ( Shoes)=>250 bacteria,> 250 of yeast,> 250 of mold. ( Table)= 1 bacteria, 0 yeast, 0 mold. The next experiment was “5 second rule”. Dry experiment, when you drop one cotton swab on the floor for 5 seconds. The results was, 3 bacteria, 9yeast, 0 mold. Wet cotton swab = 4 bacteria, 3 yeast, 0 mold This is blood agar experiment; (throat) results was hemolysis… (cough) was 0 (lips) was hemolysis. Positive control was>250 of bacteria, yeast, and 0 mold… Negative control was 0.